Odne®Fill: Biocompatibility of a Novel Light-Curable Hydrogel-Based Root Canal Obturation Material: In Vivo and In Vitro Analyses

 

Authors: Moe Sandar Kyaw PhD, MDSc, Yoshio Yahata DDS, PhD, Masato Nakano DDS, PhD, Fusami Toyama DDS, Chen Ke DDS, Wang Shuai DDS, MDSc, Yuya Kamano DDS, PhD, Futaba Harada DDS, PhD, Tomose Noguchi DDS, Masahiro Saito DDS, PhD.

 

Biocompatibility of a Novel Light-Curable Hydrogel-Based Root Canal Obturation Material: In Vivo and In Vitro Analyses

Journal of Endodontics

 

This study investigated the biocompatibility of OdneFill through both in vivo and in vitro approaches. In a live rat model, mandibular molars were treated and analyzed over 3, 28, and 90 days, comparing OdneFill to a conventional resin-based sealer and control groups. Histological and immunohistochemical evaluations revealed that OdneFill elicited significantly lower inflammatory responses and reduced infiltration of neutrophils and macrophages relative to the resin-based comparator, which showed persistent inflammation at day 90. In vitro testing using immune cell cultures demonstrated that OdneFill preserved cell viability and did not induce expression of proinflammatory cytokines. Conversely, the resin-based sealer extract reduced cell viability and upregulated inflammatory markers. These findings indicate that OdneFill offers superior biocompatibility, minimal tissue irritation, and a favorable immunological profile, supporting its potential as a next-generation material for root canal obturation.

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About the author

 

Masahiro Saito, DDS, PhD, is a Professor in the Department of Conservative Dentistry at the Tohoku University Graduate School of Dentistry and Chief of the Department of Endodontics at Tohoku University Hospital. He is also a Specialist Instructor of the Japanese Society for Conservative Dentistry. Dr. Saito has held academic and leadership roles at Kanagawa Dental College, Osaka University, and the Tokyo University of Science, and has served as Vice Director of Tohoku University Hospital. His work spans endodontics, restorative dentistry, and oral molecular immunoregulation, with extensive contributions to research, clinical practice, and academic societies in Japan.